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Table 1 Main characteristics describing the implementation and the 5 years usage (2008–2013) of 8 single-platform, volumetric, Auto40 flow cytometers for community-based point-of-care CD4 T lymphocytes enumeration installed in Chad

From: Field evaluation in Chad of community usage of CD4 T lymphocyte counting by alternative single-platform flow cytometry

   Location of Auto40 flow cytometers
Catogories Items N’Djamena (hôpital Militaire D’Instruction) N’Djamena (Centre D’Appui Psycho-Médico-Social Al-Nadjma) Mongo Bongor Kelo Doba Maïssala Koumra
  Level of laboratory facility* 1 3 2 2 3 2 3 3
Laboratory characteristics Situation Urban Urban Rural Rural Rural Urban rural Rural
  Distance from the capital city NDJ (km) 0 0 500 230 383 570 750 670
  Location in closed dust-free room (yes/no) Yes Yes No No No Yes No No
  Mean temperature gradient (minimum/maximum in °C) 22/45 22/45 22/45 20/40 20/40 20/40 20/40 22/40
  Air conditioned (yes/no) Yes No No No No Yes No  
Electricity & water Unreliable electricity power supplies No Yes Yes Yes Yes Yes Yes Yes
  Unreliable water supplies No No No No No No No No
Human resources Biologist (number) No No No No No No No No
  Trained technician (number) 1 1 1 1 1 1 1 1
Reagents & consumable supplies CD45, CD4 lyophilisated reagents Yes Yes Yes Yes Yes Yes Yes Yes
  Reagents delivery Regular Regular Regular Regular Regular Regular Regular Regular
  Reagents price (€) 10 10 12 11 11 12 12 12
  Sheath fluid recycling cassette No No Yes Yes Yes Yes Yes Yes
  Number of followed up patients (in 2012) 102 458 57 53 198 87 41 157
Medical activity in each site Number of antiretroviral drugs-treated adult patients (in 2012) 95 421 55 49 137 82 33 145
  Number of antiretroviral drugs-treated children (< 5 years) patients (in 2012) 7 37 2 4 61 5 4 12
  Number of CD4 T cells enumeration** (in 2011) 341 1156 55 123 559 230 62 358
  Final cost of CD4 T cell count (in absolute number and percentage)*** 11.0 11.0 13.0 12.0 12.1 13.1 13.4 13.2
Quality control Assurance quality procedures Yes Yes Yes Yes Yes Yes Yes Yes
  Internal quality control Yes Yes Yes Yes Yes Yes Yes Yes
  International external quality control No No No No No No No No
Instrument Support Routine maintenance on site Yes Yes Yes Yes Yes Yes Yes Yes
  Manufacturer’s maintenance (agreement) Yes Yes Yes Yes Yes Yes Yes Yes
  Troubleshooting No No Yes (electronic memory card) No No No No No
  1. *Level 1: Reference laboratory; Level 2: District laboratory; Level 3: Health care center laboratory.
  2. **Results of CD4 T cell enumeration with Auto40 flow cytometer are systematically given in absolute count as well as in percentage.
  3. ***The estimated final cost of 1 CD4 T cell enumeration does not take into account the cost of the use of sheath fluid recycling cassette in province laboratories, as well as the contractual maintenance of the Auto40 flow cytometers by the distributor (Inodia, France).